John Van Der Oost

Title: Innovations of John Van Der Oost

Introduction:

John Van Der Oost, an accomplished inventor based in Renkum, Netherlands, has significantly contributed to the field of genetic engineering with an impressive portfolio of 21 patents. His work, particularly in the development of thermostable Cas9 nucleases, represents a critical advancement in nucleic acid editing and genome modification.

Latest Patents:

Among his latest patents is the invention of Thermostable Cas9 nucleases, which involves a polynucleotide encoding a ThermoCas9 protein. This innovation enables the engineering of eukaryotic cells, such as fungi, yeast, or algae, allowing the ThermoCas9 endonuclease to be integrated and expressed from the cell's genome. By using a second expression plasmid containing an inducible promoter and a polynucleotide encoding a guide RNA, cells expressing ThermoCas9 can target and cleave DNA at specific loci or genes of interest. The invention also provides a repair-oligo that facilitates homologous recombination, leading to deletions or substitutions within the targeted DNA. Furthermore, the thermostable Cas9 nucleases described allow for genetic engineering at elevated temperatures, making them valuable tools in modern biotechnology.

Career Highlights:

Throughout his career, John Van Der Oost has worked with notable institutions, including Wageningen Universiteit and Caribou Biosciences, Inc. His dedication to research and development has positioned him as a prominent figure in genetic engineering, contributing innovative solutions that enhance the capabilities of gene editing technologies.

Collaborations:

John has collaborated with esteemed colleagues such as Stan Johan Jozef Brouns and Richard Van Kranenburg. These partnerships have fostered an environment of creativity and scientific inquiry, further advancing the field of nucleic acid editing through collaborative efforts.

Conclusion:

With 21 patents to his name, John Van Der Oost exemplifies the spirit of innovation in the realm of genetic engineering. His groundbreaking work on thermostable Cas9 nucleases not only expands the toolbox available for genetic manipulation but also opens new avenues for research and application in various biological systems. His contributions will likely influence the ongoing evolution of genome editing techniques and their practical applications in biotechnology.