John M Pryor

Title: Innovations by John M Pryor

Introduction

John M Pryor is a notable inventor based in Ipswich, MA (US). He has made significant contributions to the field of biotechnology, particularly in the area of DNA assembly. With a total of 3 patents, his work has advanced the methods used in genetic engineering and molecular biology.

Latest Patents

One of his latest patents is focused on the ordered assembly of multiple DNA fragments. This invention provides a composition and its uses, including a kit that features a synthetic self-complementary oligonucleotide. This oligonucleotide has a double-stranded region containing a binding sequence for PaqCI, along with unligatable 3' and 5′ ends that cannot be cleaved by PaqCI. The composition is designed to be combined with PaqCI or its variants in a reaction mixture, which may also include a ligase and other components for enhanced functionality.

Another significant patent by Pryor involves improved methods for the in vitro assembly of polynucleotides. This innovation enhances the frequency and fidelity of assembling large numbers of DNA fragments into a single large DNA molecule. The method utilizes a computer system that integrates ligation data to optimize synthetic overhangs for assembly by ligation. A novel restriction endonuclease is employed to create precise cuts in the DNA, allowing for more accurate assembly reactions.

Career Highlights

John M Pryor is currently associated with New England Biolabs, Inc., where he continues to contribute to advancements in biotechnology. His work has been instrumental in developing new techniques that streamline the process of DNA assembly, making it more efficient and reliable.

Collaborations

Throughout his career, Pryor has collaborated with notable colleagues such as Gregory Lohman and Vladimir Potapov. These collaborations have further enriched his research and innovations in the field.

Conclusion

John M Pryor's contributions to biotechnology through his patents and work at New England Biolabs, Inc. highlight his role as a leading inventor in the field. His innovative approaches to DNA assembly are paving the way for future advancements in genetic engineering.