John M Dunbar

Title: John M Dunbar: Innovator in Portable Sample Preparation and Analysis

Introduction

John M Dunbar is a notable inventor based in Santa Fe, NM (US). He has made significant contributions to the field of portable sample preparation and analysis, holding a total of 3 patents. His work focuses on innovative methods for characterizing micron and sub-micron particles using advanced optical techniques.

Latest Patents

One of Dunbar's latest patents is a portable sample preparation and analysis system designed for micron and sub-micron particle characterization using light scattering and absorption spectroscopy. This invention provides a method and device for remote sampling, preparation, and optical interrogation of samples. The portable device utilizes a filtration-based approach to remove interfering background particles from the sample matrix, allowing for accurate analysis of the chosen analyte. The device is capable of calculating the initial concentration of the analyte and diluting it for reliable optical measurements. Suitable analytes include cells, microorganisms, bioparticles, pathogens, and diseases, with sample matrices ranging from biological fluids like blood and urine to environmental samples such as wastewater.

Another significant patent involves the preparation of DNA-containing extracts for PCR amplification. This method addresses the challenge of impurities in environmental samples that can interfere with PCR amplification and DNA quantitation. By lysing cells from samples of soil, river water, and aerosol, Dunbar's method releases DNA into an aqueous buffer. The process includes treating the extract with N-phenacylthiazolium bromide to precipitate impurities, resulting in a supernatant that can be used for PCR amplification within 5–10 minutes.

Career Highlights

Throughout his career, John M Dunbar has worked with prestigious organizations, including the University of California and Los Alamos National Security, LLC. His experience in these institutions has contributed to his expertise in the field of sample analysis and preparation.

Collaborations

Dunbar has collaborated with notable colleagues such as Cheryl R Kuske and Peter C Stark. Their combined efforts have further advanced the research and development of innovative analytical techniques.

Conclusion

John M Dunbar's contributions to portable sample preparation and analysis have made a significant impact in the field. His innovative patents and collaborations highlight his dedication to advancing scientific research and technology.