John L Humes

Title: The Innovative Contributions of John L. Humes

Introduction

John L. Humes is a notable inventor based in Berkeley Heights, NJ (US). He has made significant contributions to the field of immunoassays, particularly in the detection of human polymorphonuclear leukocyte elastase activity. With a total of 3 patents to his name, Humes has demonstrated a commitment to advancing medical diagnostics.

Latest Patents

Humes' latest patents include an assay for a marker of human polymorphonuclear leukocyte elastase activity. This immunoassay is based on the detection of leukocyte-elastase produced fibrinogen cleavage peptides. It allows for the evaluation of the potency of compounds that inhibit the formation of these cleavage peptides in various in vitro cell biological situations. The assay can detect an endogenous leukocyte-elastase produced fibrinogen cleavage peptide signal in normal human plasma and at elevated levels in cystic fibrosis plasma and rheumatoid arthritis synovial fluid samples. The procedure can be a single-step assay, enabling rapid and reproducible detection of specific cleavage peptides.

Another significant patent is for evaluating the inhibition of polymorphonuclear leukocyte elastase. Humes has identified N-substituted azetidinones as a class of inhibitors of human leukocyte elastase, which are useful in treating a wide variety of anti-inflammatory and anti-degenerative diseases. The therapeutic agents form a stable complex with the enzyme, and the assay disclosed allows for the hydrolysis of this inhibitor-enzyme complex, measuring specific products of the hydrolysis. These assays are beneficial in clinical settings for determining appropriate dosages and assessing treatment effectiveness.

Career Highlights

Humes is currently associated with Merck & Company, Inc., where he continues to contribute to innovative research and development in the pharmaceutical industry. His work has had a significant impact on the field of immunology and therapeutic development.

Collaborations

Throughout his career, Humes has collaborated with notable colleagues, including Richard A. Mumford and William A. Hanlon. These collaborations have further enhanced the quality and reach of his research.

Conclusion

John L. Humes is a distinguished inventor whose work in immunoassays has paved the way for advancements in medical diagnostics. His contributions continue to influence the field and improve patient care.