Location History:
- South Dennis, MA (US) (1997)
- Brewster, MA (US) (1996 - 1998)
Company Filing History:
Years Active: 1996-1998
Title: Innovations of John K Eldredge
Introduction
John K Eldredge is a notable inventor based in Brewster, MA (US). He has made significant contributions to the field of nucleic acid hybridization, holding a total of 3 patents. His work has advanced the methods used in detecting target nucleic acids, showcasing his expertise and innovative spirit.
Latest Patents
Eldredge's latest patents include the development of hybridization promotion reagents. These novel reagents are designed for use in nucleic acid hybridization assays, facilitating the detection of target nucleic acids in various samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate, which enhances hybridization between target nucleic acids and probes. This innovation surprisingly results in the phenomenon of superstoichiometric labeling. Additionally, he has developed methods for improving the sensitivity of hybridization assays. These methods aim to reduce non-specific binding (NSB) and non-specific hybridization (NSH). They include a washing method utilizing tetraalkylammonium salts at high temperatures, as well as release methods that allow for the recapture of probe-target complexes from solid supports. The combination of these techniques leads to a substantial reduction in NSB and NSH without the need for multiple rounds of release and recapture.
Career Highlights
Eldredge is currently associated with Amoco Corporation, where he continues to innovate and contribute to the field. His work has not only advanced scientific understanding but has also practical applications in various industries.
Collaborations
Throughout his career, Eldredge has collaborated with notable colleagues, including Mark L Collins and Kevin R Kearney. These partnerships have fostered a collaborative environment that enhances the development of innovative solutions.
Conclusion
John K Eldredge's contributions to the field of nucleic acid hybridization are significant and impactful. His innovative patents and collaborative efforts continue to shape advancements in this area of research.