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Title: John F Uher: Innovator in Recombinant Protein Production
Introduction
John F Uher is a notable inventor based in Kenosha, WI (US). He has made significant contributions to the field of biotechnology, particularly in the production of recombinant proteins. With a total of 3 patents to his name, Uher's work has implications for diagnostic assays and the understanding of intrinsic factors.
Latest Patents
One of Uher's latest patents focuses on the cloning, expression, and purification of recombinant porcine intrinsic factor for use in diagnostic assays. This invention relates to a novel method of producing functionally active recombinant porcine intrinsic factor, which is crucial for various biological applications. The method involves introducing a vector containing DNA encoding the protein into a Chinese Hamster Ovary (CHO) host cell, optimizing conditions for the expression of functional protein. The resulting protein can achieve a purity level of at least 97%. Additionally, Uher's patent includes an isolated nucleotide sequence encoding the porcine intrinsic factor, which can be utilized in assays to determine vitamin levels in biological samples.
Career Highlights
Throughout his career, John F Uher has worked with prominent organizations, including Abbott Laboratories Corporation. His expertise in recombinant protein production has positioned him as a valuable contributor to advancements in biotechnology.
Collaborations
Uher has collaborated with notable colleagues such as Ramani S Wonderling and Thomas P Leary. These partnerships have further enhanced his research and development efforts in the field.
Conclusion
John F Uher's innovative work in recombinant protein production exemplifies the impact of inventors in advancing scientific knowledge and technology. His contributions continue to influence the field of biotechnology and diagnostic assays.