Jiakai Cui

Title: Jiakai Cui: Innovator in DNA Synthesis and Assembly

Introduction

Jiakai Cui is a prominent inventor based in Wuhan, China. He has made significant contributions to the field of biotechnology, particularly in the efficient synthesis and assembly of large fragment DNA. His innovative methods have the potential to streamline genetic engineering processes and enhance research capabilities.

Latest Patents

Jiakai Cui holds a patent for an "Efficient synthesis and assembly method for large fragment DNA based on programmable nuclease Argonaute." This method involves constructing and treating antibiotic resistance gene reconstructed vectors with linearization. It includes dividing a target DNA into multiple small DNA fragments, synthesizing these fragments, and loading them onto the antibiotic resistance gene reconstructed vectors. The method utilizes SLIC and resistance gene reconstruction to achieve the assembly of the target DNA. This approach allows for the assembly of 5-6 small fragments in a single reconstruction, making it more efficient and time-saving. Additionally, it avoids introducing mutations caused by PCR, eliminating the need for a second sequencing, which saves both time and costs.

Career Highlights

Jiakai Cui is affiliated with Hubei University, where he continues to advance his research in DNA synthesis and assembly. His work has garnered attention for its innovative approach and practical applications in genetic engineering.

Collaborations

Jiakai Cui collaborates with notable colleagues, including Lixin Ma and Wanping Chen. Their combined expertise contributes to the advancement of research in their field.

Conclusion

Jiakai Cui's contributions to DNA synthesis and assembly represent a significant advancement in biotechnology. His innovative methods not only enhance efficiency but also reduce costs in genetic engineering processes. His work continues to inspire future research and innovation in the field.