Jeffrey G Thomas

Title: Innovations by Jeffrey G Thomas

Introduction

Jeffrey G Thomas is an accomplished inventor based in Bellevue, WA (US). He has made significant contributions to the field of binding proteins, with a focus on developing innovative techniques for isolating these proteins with high affinity to ligands. His work has led to advancements that address the limitations of previous methods.

Latest Patents

Jeffrey G Thomas holds 2 patents. His latest patent involves the isolation of binding proteins with high affinity to ligands. This invention provides a rapid approach for isolating binding proteins capable of binding small molecules and peptides through 'display-less' library screening. In this technique, libraries of candidate binding proteins, such as antibody sequences, are expressed in soluble form in the periplasmic space of gram-negative bacteria. These proteins are then mixed with a labeled ligand. Clones expressing recombinant polypeptides with affinity for the ligand show an increased concentration of the labeled ligand bound to the binding protein, allowing for the isolation of the cells from the rest of the library. When fluorescent labeling of the target ligand is utilized, cells can be isolated by fluorescence-activated cell sorting (FACS). This approach is more rapid than prior art methods and avoids issues associated with the surface expression of ligand fusion proteins used in phage display.

Career Highlights

Jeffrey G Thomas is affiliated with the University of Texas System, where he continues to contribute to research and innovation in his field. His work has garnered attention for its practical applications and efficiency in isolating binding proteins.

Collaborations

Some of his notable coworkers include Gang Chen and Andrew Hayhurst, who have collaborated with him on various projects related to binding proteins and their applications.

Conclusion

Jeffrey G Thomas is a notable inventor whose work in isolating binding proteins has led to significant advancements in the field. His innovative techniques are paving the way for more efficient methods in protein research and applications.