Janice Marie Cline

Title: Janice Marie Cline: Innovator in Multi-Site Mutagenesis

Introduction

Janice Marie Cline is a prominent inventor based in San Marcos, CA (US). She has made significant contributions to the field of biotechnology, particularly in the area of mutagenesis. With a total of 4 patents, her work has advanced the methods used in genetic engineering.

Latest Patents

One of her latest patents focuses on multi-site mutagenesis. This invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The compositions and methods developed by Cline increase mutation frequency and the number of transformants. This allows researchers to sequence only a few clones to identify the correct mutants. Consequently, it enables the desired mutant to be obtained by screening a large number of transformants in a short time. Additionally, the inclusion of FEN-1, PEF, and optimized buffer and cycling conditions facilitates random mutagenized library construction and the mutagenesis of large or difficult templates.

Career Highlights

Throughout her career, Janice has worked with notable companies such as Agilent Technologies, Inc. and Stratagene California. Her experience in these organizations has contributed to her expertise in the field of biotechnology.

Collaborations

Janice has collaborated with several professionals in her field, including Holly Hogrefe and Connie Jo Hansen. These collaborations have further enriched her work and innovations.

Conclusion

Janice Marie Cline is a distinguished inventor whose contributions to multi-site mutagenesis have had a significant impact on genetic engineering. Her innovative methods and collaborations continue to influence the field positively.