Company Filing History:
Years Active: 1998-2002
Title: Innovations of James Leushner
Introduction
James Leushner is a prominent inventor based in North York, Canada. He has made significant contributions to the field of genetics and molecular biology, holding a total of 10 patents. His work focuses on innovative methods for sequencing nucleic acids and testing for genetic mutations.
Latest Patents
One of his latest patents is a method for sequencing nucleic acid polymers. This method allows for the sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample within a single vessel. The process involves combining the sample with a sequencing mixture that includes a primer pair, a thermally stable polymerase, nucleotide feedstocks, and a chain-terminating nucleotide. The reaction mixture also incorporates an unconventional nucleotide and an appropriate enzyme for degradation of nucleic acid polymers containing this unconventional nucleotide. The mixture undergoes multiple thermal cycles for annealing, extension, and denaturation, ultimately producing a product mixture that is analyzed by electrophoresis.
Another significant patent by Leushner is related to testing for mutations in the BRCA1 gene. This method employs a hierarchical approach where samples are amplified in one or more multiplex PCR amplification reactions. The resulting mixtures of amplified fragments are evaluated and compared to standard values from the wild-type BRCA1 gene. Differences in fragment sizes and amounts indicate potential mutations. For samples where no mutation is detected, further sequencing of the BRCA1 gene exons is performed.
Career Highlights
James Leushner is currently associated with Visible Genetics Inc., a company that specializes in genetic testing technologies. His innovative work has positioned him as a key figure in the field of genetic research and diagnostics.
Collaborations
Leushner has collaborated with notable colleagues, including James M. Dunn and May Hui, contributing to advancements in genetic testing methodologies.
Conclusion
James Leushner's contributions to the field of genetics through his innovative patents and collaborations have significantly advanced the understanding and testing of genetic mutations. His work continues to impact the landscape of molecular biology and genetic diagnostics.
Inventor’s Patent Attorneys refers to legal professionals with specialized expertise in representing inventors throughout the patent process. These attorneys assist inventors in navigating the complexities of patent law, including filing patent applications, conducting patent searches, and protecting intellectual property rights. They play a crucial role in helping inventors secure patents for their innovative creations.