James E Brown

Title: Innovations and Contributions of Inventor James E Brown

Introduction

James E Brown is a notable inventor based in Lafayette, CA (US). He has made significant contributions to the field of immunology and biochemistry, holding 2 patents that showcase his innovative approach to solving complex scientific problems.

Latest Patents

One of his latest patents is an immunoassay for cell proteins. This immunoassay is designed to detect cellular proteins that may contaminate products purified from mammalian cell cultures. Given that mammalian cell culture utilizes protein-containing media, the assay is specifically tailored to recognize cellular proteins while excluding media proteins. This specificity is achieved through the selective adsorption of an antiserum to cellular proteins against media proteins. The quantification of this assay can be enhanced by using a purified antigen, such as fibronectin, which is known for its high immunogenicity.

Another significant patent by James E Brown is related to the phospholipid affinity purification of Factor VIII:C. This method involves the affinity purification of Factor VIII:C, whether derived from plasma or genetically engineered, using coupled phosphatidylserine (PS) as the primary phospholipid. This technique results in a high degree of purity for Factor VIII:C, comparable to that achieved with monoclonal antibodies specific to either Factor VIII:C or von Willebrand factor. Additionally, phospholipids such as phosphatidylcholine (PC) and phosphatidylethanolamine (PE) can be utilized in conjunction with PS.

Career Highlights

Throughout his career, James E Brown has worked with reputable companies, including Miles Laboratories, Inc. and Miles Inc. His work in these organizations has contributed to advancements in the field of biochemistry and immunology.

Collaborations

James E Brown has collaborated with various professionals in his field, including Cynthia A Cowgill. These collaborations have further enriched his research and innovations.

Conclusion

James E Brown's contributions to the field of immunology through his patents and career achievements highlight his role as a significant inventor. His innovative approaches continue to influence the scientific community and pave the way for future advancements.