Location History:
- Livermore, CA (US) (2002 - 2004)
- Novi, MN (US) (2006)
Company Filing History:
Years Active: 2002-2006
Title: Innovations by James D. Tucker
Introduction
James D. Tucker is a notable inventor based in Livermore, CA (US). He has made significant contributions to the field of biotechnology, particularly in the area of DNA sequencing and amplification. With a total of 3 patents, his work has advanced the understanding and application of genetic materials.
Latest Patents
One of his latest patents involves derivatized versions of ligase enzymes for constructing DNA sequences. This method allows for the creation of very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided, which are sequentially hybridized to each other. The enzymatic ligation of these oligonucleotides results in a contiguous piece of PCR-ready DNA of predetermined sequence.
Another significant patent focuses on rolling circle amplification detection of RNA and DNA. This technique has proven useful for detecting point mutations in isolated nucleic acids, although its application in cytological preparations has faced challenges. By pretreating cells with a combination of restriction enzymes and exonucleases, Tucker demonstrates RCA in solution and in situ to detect gene copy number and single base mutations. This method can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.
Career Highlights
James D. Tucker is affiliated with the University of California, where he continues to innovate and contribute to the scientific community. His work has garnered attention for its practical applications in genetic research and diagnostics.
Collaborations
He has collaborated with notable colleagues, including Allen T. Christian and Melissa S. Pattee, further enhancing the impact of his research.
Conclusion
James D. Tucker's contributions to biotechnology through his patents and collaborations highlight his role as a leading inventor in the field. His innovative methods for DNA construction and amplification are paving the way for future advancements in genetic research.
Inventor’s Patent Attorneys refers to legal professionals with specialized expertise in representing inventors throughout the patent process. These attorneys assist inventors in navigating the complexities of patent law, including filing patent applications, conducting patent searches, and protecting intellectual property rights. They play a crucial role in helping inventors secure patents for their innovative creations.