Company Filing History:
5 out of 33 
5 out of 71 Years Active: 2022-2024
Title: Innovations in Genetic Engineering by Ioannis Mougiakos
Introduction
Ioannis Mougiakos is a prominent inventor based in Wageningen, Netherlands. He has made significant contributions to the field of genetic engineering, particularly in the development of novel tools for nucleic acid editing and genome modification. With a total of five patents to his name, his work is paving the way for advancements in biotechnology.
Latest Patents
One of his latest patents involves thermostable Cas9 nucleases. This invention utilizes a polynucleotide encoding a ThermoCas9 protein along with a constitutive promoter to engineer eukaryotic cells, such as fungi, yeast, or algae. The ThermoCas9 endonuclease is integrated and expressed from the genome of the cell. A second expression plasmid is then used to transfect these cells, containing an inducible promoter and a polynucleotide encoding a guide RNA. The guide RNA pairs with the ThermoCas9 to facilitate targeted endonuclease activity, cleaving the cell DNA at a specific locus or gene of interest. Additionally, a repair-oligo is provided to the cell, enabling homologous recombination following DNA cleavage, which results in either a deletion or substitution of nucleotides at the targeted site. This innovative approach allows for gene editing at elevated temperatures, enhancing the efficiency of genetic modifications.
Career Highlights
Ioannis Mougiakos has worked with notable institutions such as Wageningen University and the Foundation for Technical Sciences. His research and inventions have significantly impacted the field of genetic engineering, particularly in the development of tools that operate effectively at higher temperatures.
Collaborations
Throughout his career, Mougiakos has collaborated with esteemed colleagues, including John Van Der Oost and Richard Van Kranenburg. These partnerships have further enriched his research and contributed to the advancement of genetic engineering technologies.
Conclusion
Ioannis Mougiakos is a key figure in the realm of genetic engineering, with his innovative patents and collaborations driving progress in the field. His work continues to influence the future of biotechnology and genetic modifications.
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