Hui-Lee Wong

Title: **Innovator Hui-Lee Wong: Advancing DNA Methylation Analysis**

Introduction

Hui-Lee Wong is a notable inventor based in South Pasadena, California, who has made significant contributions to the field of genetic research. With a patent to his name, Wong's work focuses on innovative methods that enhance our understanding of DNA methylation, which plays a crucial role in gene regulation and expression.

Latest Patents

Hui-Lee Wong holds a patent for a "High throughput method of DNA methylation haplotyping." This innovative method offers new, high-throughput techniques to quantify DNA methylation with precision, particularly at a single-base resolution. The approach centers around the use of allele-specific sequence polymorphisms in proximity to CpG methylation sites, enabling researchers to distinguish between the methylation levels of different alleles. Key steps in this methodology include bisulfite modification, PCR amplification, and subsequent allele-specific pyrosequencing to determine the percentage of DNA methylation based on cytosine to thymidine conversion ratios. This inventive technique holds substantial utility in analyzing X-inactivation, allele-specific gene expression related to the immune system, and identifying loss of imprinting.

Career Highlights

Wong is affiliated with the University of Southern California, where he engages in advanced research and applications of his patented methodologies. His contributions have provided insights into complex genetic mechanisms, thereby enhancing the understanding of various biological processes.

Collaborations

Throughout his career, Hui-Lee Wong has collaborated with esteemed colleagues such as Mihaela Campan and Peter W. Laird. These partnerships highlight the collaborative nature of scientific research, allowing for the pooling of expertise and resources to drive innovation in the field of genetics.

Conclusion

Hui-Lee Wong's work exemplifies the impact of innovative thinking in the realms of genetics and molecular biology. His patent for a high-throughput method of DNA methylation haplotyping not only showcases his ingenuity but also underscores the importance of collaboration in the pursuit of scientific advancement. Through his contributions, Wong continues to pave the way for future discoveries that may further unravel the complexities of genetic regulation.