Company Filing History:
Years Active: 2014-2019
Title: Holger Wedler: Innovator in Nucleic Acid Technologies
Introduction
Holger Wedler is a prominent inventor based in Hilden, Germany. He has made significant contributions to the field of molecular biology, particularly in the area of nucleic acid detection and purification. With a total of 3 patents to his name, Wedler's work has advanced the methodologies used in quantitative real-time polymerase chain reaction (qPCR) and nucleic acid isolation.
Latest Patents
Wedler's latest patents include innovative methods for nucleic acid detection and quantification. One of his notable inventions relates to the detection or quantification of newly-synthesized double-stranded target nucleic acid molecules during qPCR amplification. This invention utilizes an intercalating dye that recognizes double-stranded DNA with higher affinity than single-stranded DNA, along with a fluorophore-labeled oligonucleotide probe that is sequence-specific for the target nucleic acid. This dual approach enables the quantification of specific targets and the total amount of mixed nucleic acid populations, while also assessing the causes of suboptimal PCR performance.
Another significant patent involves a chromatographic device designed for isolating and purifying double-stranded nucleic acids, particularly double-stranded DNA. This invention addresses the need for effective separation from single-stranded nucleic acids, oligonucleotides, mononucleotides, salts, and other impurities. The accompanying method and kit for chromatographic isolation further enhance the utility of this invention in laboratory settings.
Career Highlights
Holger Wedler is currently associated with Qiagen GmbH, a leading company in the field of molecular diagnostics and sample preparation. His work at Qiagen has allowed him to focus on developing cutting-edge technologies that facilitate nucleic acid research and applications.
Collaborations
Throughout his career, Wedler has collaborated with notable colleagues, including Jan Petzel and Roland Fabis. These collaborations have contributed to the advancement of his research and the successful development of his patented technologies.
Conclusion
Holger Wedler's contributions to nucleic acid technologies have significantly impacted the field of molecular biology. His innovative patents and work at Qiagen GmbH continue to influence research and applications in nucleic acid detection and purification.