Company Filing History:
Years Active: 2010-2012
Title: Innovations of Hiroaki Torii in Peptide Analysis
Introduction
Hiroaki Torii is a notable inventor based in Tokyo, Japan. He has made significant contributions to the field of peptide analysis, holding three patents that showcase his innovative methods. His work primarily focuses on improving the techniques used to analyze the C-terminal amino acid sequences of peptides.
Latest Patents
One of Hiroaki Torii's latest patents is a method for analyzing the C-terminal amino acid sequence of a peptide using mass spectrometry. This invention provides a technique for successively releasing the C-terminal amino acids of a peptide while minimizing undesirable side reactions, such as the cleavage of peptide bonds in intermediate positions. The method involves subjecting a dry sample of a long amino acid peptide to an N-acylation treatment. By utilizing a reaction reagent that combines an alkanoic acid anhydride with a small amount of a perfluoroalkanoic acid, the successive release of C-terminal amino acids is conducted under mild conditions. Following this, a hydrolysis treatment is applied, and selective fragmentization at the site of the arginine residue is performed through trypsin digestion. The molecular weight decreases of the C-terminal side fragments are then measured using a MALDI-TOF-MS apparatus, allowing for the identification of the C-terminal amino acid sequence of the peptide sample.
Career Highlights
Hiroaki Torii is currently employed at NEC Corporation, where he continues to develop innovative methods in peptide analysis. His work has garnered attention in the scientific community, contributing to advancements in biochemistry and molecular biology.
Collaborations
Hiroaki has collaborated with notable coworkers, including Kenji Miyazaki and Akira Tsugita, who have also contributed to the field of peptide analysis.
Conclusion
Hiroaki Torii's innovative methods in peptide analysis have significantly advanced the understanding of amino acid sequences. His contributions through patents and collaborations highlight the importance of innovation in scientific research.