Nec Corporation

Hiroaki Torii

Kenichi Kamijo

Kenji Miyazaki

Akira Tsugita

Keiichi Yoshida

Tomoharu Kiyuna

Kenji Okajima

Masahiko Kuroda

Kiyoshi Mukai

Ken' Ichi Kamijo

The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such a undesirable side reaction as cleavage of peptide bond in the intermediate position of the peptide and can carry out the chemical treatment thereof under widely applicable conditions; In the method, a dry sample of a peptide with long amino acid length is beforehand subjected to an N-acylation treatment; by using a reaction reagent where an alkanoic acid anhydride is combined with a small amount of a perfluoroalkanoic acid, successive release of C-terminal amino acids is conducted under mild conditions; a hydrolysis treatment is applied; then, selective fragmentization at site of arginine residue is performed by digestion by trypsin; thereafter, decreases in molecular weight are measured for the C-terminal side fragments derived from a series of reaction products with use of a MALDI-TOF-MS apparatus; thereby, the C-terminal amino acid sequence of the peptide sample is identified.

Method for analyzing C-terminal amino acid sequence of peptide using mass spectrometry

A method of evaluating a cell state based on information of an image taken of a cell containing a chromosome territory is provided. This method includes extracting the chromosome territory from the image (S), standardizing a positioning state of the chromosome territory and then quantifying the positioning state (S), and evaluating the cell state based on the quantified positioning state of the chromosome territory (S).

Method of evaluating chromosome state and evaluation system

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Tokyo, Japan

Hiroaki Torii