Hilde E Smith

Introduction

Hilde E. Smith is a notable inventor based in Groningen, Netherlands, with a significant contribution to the field of protein processing. With three patents to her name, Smith has been at the forefront of advancements in biotechnology, focusing specifically on methods and systems to enhance protein expression and secretion.

Latest Patents

One of her latest patents revolves around the **Processing of Proteins**, detailing the cloning and overexpression of leader peptidase genes. This invention describes a method for isolating a leader peptidase gene, where the overexpression of the signal peptidase in a compatible host species leads to an enhanced rate of protein processing.

Another significant contribution from Smith includes her patent on **Secretory Signal Selection Vectors for Extracellular Protein Synthesis**. This innovation presents novel vectors that facilitate the identification of secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids contain a multiple cloning site with restriction sites in reading frame with a structural gene, enabling rapid screening of the secreted expression product. These vectors may also feature a promoter region upstream from the multiple cloning site, optimized for the bacterium Bacillus. This invention has improved upon previously known sequences, allowing for efficient protein secretion into the supernatant, thus providing economically high yields of proteins.

Career Highlights

Hilde E. Smith currently works at Gist-Brocades N.V., a company recognized for its innovative contributions to biotechnology and the production of enzymes. Her role at the company aligns with her research interests, allowing her to apply her inventions in practical, industrial applications.

Collaborations

Smith has collaborated with esteemed colleagues such as Sierd Bron and Gerard Venema. These partnerships have furthered her research efforts and contributed to the development of her innovative solutions in protein processing.

Conclusion

Hilde E. Smith exemplifies the spirit of innovation in her field. Through her patents, she has made significant strides in protein processing and secretion technologies, showcasing her expertise and dedication to advancing biotechnological applications. Her work at Gist-Brocades N.V. and her collaborations with other skilled professionals underline her influential role in the research community.