Guoxian Liu

Title: Guoxian Liu: Innovator in DNA Polymerase Technology

Introduction

Guoxian Liu is a prominent inventor based in Beijing, China. He has made significant contributions to the field of biotechnology, particularly in the development of DNA polymerases. With a total of 2 patents, Liu's work has the potential to impact various applications in genetic research and biotechnology.

Latest Patents

Liu's latest patents include a groundbreaking DNA polymerase that possesses continuous catalytic capacity and salt tolerance. This DNA polymerase features an amino acid sequence represented by SEQ ID NO:2, or a derivative of this sequence achieved through substitution, deletion, or addition of at least one amino acid residue. The innovative aspect of this polymerase lies in its hybrid nature, which is created by inserting a thioredoxin binding domain (TBD) from bacteriophage T7 DNA polymerase into a DNA polymerase I (Sau). The method for preparing this DNA polymerase involves several steps, including determining a corresponding position and target substitution sequence in the Sau protein, synthesizing a primer based on the gene sequence of Sau, cloning the Sau-TBD segment into an expression vector, and inducing protein expression.

Career Highlights

Throughout his career, Guoxian Liu has worked with notable companies in the biotechnology sector, including Hangzhou Zhongce Bio-sci & Tech. Co., Ltd. and Hangzhou Zhongee Bio-Sci & Tech. Co., Ltd. His experience in these organizations has contributed to his expertise in the development of advanced biotechnological solutions.

Collaborations

Liu has collaborated with esteemed colleagues such as Joseph Chow and Qi Cheng. These partnerships have further enriched his research and development efforts in the field of DNA polymerases.

Conclusion

Guoxian Liu's innovative work in DNA polymerase technology showcases his dedication to advancing biotechnology. His contributions are poised to have a lasting impact on genetic research and related fields.