This inventor holds 3 USPTO granted patents. Top assignee: Bio-Research Products, Inc.. Active years: 1983-1996.
Location History:
- Troy, NY (US) (1983)
- Iowa City, IA (US) (1995 - 1996)
Company Filing History:
2 out of 2 Years Active: 1983-1996
Title: George F Johnson: Innovator in Kinetic Enzyme Assays
Introduction
George F Johnson is a notable inventor based in Iowa City, IA (US). He has made significant contributions to the field of biochemistry, particularly in the development of innovative assays for measuring biochemical parameters in body fluids. With a total of 3 patents to his name, Johnson's work has the potential to enhance diagnostic techniques in medical laboratories.
Latest Patents
One of Johnson's latest patents is titled "Kinetic enzyme assay for determining the CO.sub.2 content of body fluids." This improved kinetic assay allows for the spectrophotometric determination of dissolved carbon dioxide (CO.sub.2) content in body fluids such as blood, plasma, or serum. The assay operates at approximately pH 8.0, where CO.sub.2 is primarily present as bicarbonate ion (HCO.sub.3.sup.-). The process involves a coupled reaction mixture containing phosphoenolpyruvate (PEP) and phosphoenolpyruvate carboxylase (PEPC), which catalyzes the conversion of HCO.sub.3.sup.- to oxaloacetate (OA). Subsequently, the OA is subjected to another reaction mixture with nicotinamide adenine dinucleotide (NADH) and malate dehydrogenase (MDH), resulting in the reduction of OA to malate and the oxidation of NADH to NAD.sup.+. The CO.sub.2 level in the sample can be indirectly determined by measuring the change in NADH concentration. An inhibitor is added to the reaction mixture to ensure that the reaction kinetics remain essentially first order across the relevant bicarbonate concentration range.
Career Highlights
George F Johnson has been associated with Bio-Research Products, Inc., where he has applied his expertise in biochemistry to develop innovative solutions. His work has contributed to advancements in the field, particularly in the area of enzyme assays.
Collaborations
Johnson has collaborated with notable colleagues such as Bryce A Cunningham and Rubio R Punzalan, further enhancing the impact of his research and innovations.
Conclusion
George F Johnson's contributions to the field of biochemistry, particularly through his patented innovations, demonstrate his commitment to advancing medical diagnostics. His work continues to influence the development of more accurate and efficient assays for clinical applications.