Geoffrey N Roth

Title: Innovations of Geoffrey N Roth

Introduction

Geoffrey N Roth is a notable inventor based in Goshen, IN (US). He has made significant contributions to the field of bacteriophage research, holding a total of 6 patents. His work focuses on methodologies that enhance the detection and manipulation of bacteriophages, which are viruses that infect bacteria.

Latest Patents

One of his latest patents is a methodology for the detection, enumeration, propagation, and manipulation of bacteriophages. This innovative method allows for the propagation, enumeration, and quantification of bacteriophages in water samples or other aqueous environments. The method includes ingredients that stimulate the growth of specific bacterial species susceptible to infection by certain bacteriophages, while inhibiting or rendering inconsequential any interfering background organisms. Key features of the medium involve oxidation-reduction compounds that produce colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature-independent gelling agents. A preferred combination includes growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and suitable gelling agents. This combination results in a dark red bacterial lawn with teal blue-green, irregularly circular spots that represent individual phage plaques, all visible under standard lighting conditions. Additionally, the procedure can be adapted for automatic counting systems under artificial illumination and can be applied to water samples and elution buffers that retain bacteriophages in suspension after contact with contaminated solids.

Another significant patent involves a method utilizing enzyme substrates that produce insoluble fluorescent products and chromogens. This method detects cells, microorganisms, or molecules through various combinations of fluorogens and chromogens that yield fluorophores and chromophores when cleaved by specific enzymes. This innovative approach allows for the visualization of these compounds under UV and visible light.

Career Highlights

Geoffrey has worked with several companies, including Micrology Laboratories, LLC and Micrology Methods, LLC. His experience in these organizations has contributed to his expertise in bacteriophage research and detection methodologies.

Collaborations

Geoffrey has collaborated with notable individuals such as Jonathan N Roth and James Larkin, further enhancing his research and development efforts in the field.

Conclusion

Geoffrey N Roth's