Company Filing History:
Years Active: 1998-1999
Title: Frank P Diet: Innovator in Nucleotide Delivery Systems
Introduction
Frank P Diet is a notable inventor based in Koln, Germany. He has made significant contributions to the field of gene therapy and nucleotide delivery systems. With a total of 2 patents, his work focuses on innovative methods for delivering nucleic acids into living cells.
Latest Patents
One of his latest patents is titled "Intracellular delivery of nucleic acids using pressure." This invention describes a method where naked nucleic acids, drugs, and other molecules can enter cells in living intact tissue upon the application of pressure. The nucleic acids are localized to the cell nuclei, achieving transfection efficiencies greater than 90% for naked DNA and RNA. The invention utilizes a sealed enclosure that contains the cells and their extracellular environment, which is pressurized to an incubation pressure on the order of atmospheres. This method has potential applications in gene therapy, including ex-vivo treatment of grafts prior to transplantation and in-vivo treatment of various tissues.
Another significant patent by Frank P Diet is the "Apparatus and method for delivering a nucleotide into cell nuclei." This system enables cells in living intact tissue to accept nucleotides from their extracellular environment and localize them into their nuclei. The invention relies on high pressure to facilitate the uptake of nucleotides, achieving a transfection rate of over 90% in some cases. Various techniques are employed for pressurization, including enclosing tissue in an impermeable sheath and injecting fluid to reach high pressure.
Career Highlights
Frank P Diet is affiliated with Leland Stanford Junior University, where he continues to advance research in gene therapy and nucleotide delivery systems. His innovative approaches have the potential to revolutionize treatments for various medical conditions.
Collaborations
He has collaborated with notable colleagues, including Michael J Mann and Victor J Dzau, contributing to the advancement of research in his field.
Conclusion
Frank P Diet's work in nucleotide delivery systems showcases his innovative spirit and dedication to advancing gene therapy. His patents reflect a commitment to improving medical treatments through cutting-edge technology.