This inventor holds 1 USPTO granted patent. Top assignee: Texas Tech University System. Active years: 2003.
Company Filing History:
Years Active: 2003
Title: Innovations in Plant Genetic Transformation by Ellen B Peffley
Introduction
Ellen B Peffley is a notable inventor based in Lubbock, TX (US), recognized for his contributions to the field of plant genetic transformation. His innovative work focuses on utilizing the pollen tube pathway for the direct transformation of higher plants. This groundbreaking approach has the potential to revolutionize agricultural practices and enhance plant breeding techniques.
Latest Patents
Ellen B Peffley holds a patent for the "Direct transformation of higher plants through pollen tube pathway." This patent presents a genetic transformation system that employs plasmid DNA applied to the surface of an ovary wound site after the removal of the style of florets following pollination. The research demonstrated that the plasmid DNA could reach the ovules of decapitated florets within approximately 24 hours after application. PCR analyses of genomic DNA revealed that 12% to 15% of the tested plants contained a specific fragment of the luciferase gene construct integrated into their genomes. Southern blotting further confirmed the incorporation of the firefly luciferase gene construct into the genomic DNA of the plants. Notably, some PCR positive plants maintained the luciferase gene fragment in their progenies, indicating the potential for stable transformation through this method.
Career Highlights
Ellen B Peffley is affiliated with the Texas Tech University System, where he has made significant strides in plant biotechnology. His research has not only contributed to the understanding of genetic transformation but has also paved the way for future innovations in the field.
Collaborations
Ellen has collaborated with esteemed colleagues such as Randy Dale Allen and Ping Song, further enhancing the impact of his research through teamwork and shared expertise.
Conclusion
Ellen B Peffley's work in plant genetic transformation through the pollen tube pathway represents a significant advancement in agricultural biotechnology. His innovative approach holds promise for the future of plant breeding and genetic engineering.