Company Filing History:
10 out of 25 
9 out of 730 
Years Active: 1991-2000
Title: Dwane E Wylie: Innovator in Protein Purification and Metal Binding Polypeptides
Introduction
Dwane E Wylie is a prominent inventor based in Lincoln, NE (US), known for his significant contributions to the fields of protein purification and metal binding polypeptides. With a total of 15 patents to his name, Wylie has made remarkable advancements that have implications in various scientific and medical applications.
Latest Patents
Wylie's latest patents include innovative technologies such as a purification cloning method for peptides. This invention focuses on a protein purification construct that features three tandem, coupled segments composed of a binding protein, an interconnecting linker, and a variable fused polypeptide. The binding protein can be a mammalian or human carbonic anhydrase, or a modified version of it. This construct is designed for the expression of product peptides in microbial and higher organisms, as well as for ligand immobilized affinity purification of these peptides. Another notable patent involves metal binding polypeptides that include an amino acid sequence coding for a light chain variable region of a monoclonal antibody. This antibody is capable of immunoreacting with a lead cation, and the invention also encompasses fusion proteins and Fab fragments that incorporate the light chain variable region.
Career Highlights
Throughout his career, Dwane E Wylie has worked with notable organizations such as Bionebraska, Inc. and the University of Nebraska. His work in these institutions has allowed him to collaborate with other experts in the field and contribute to groundbreaking research.
Collaborations
Wylie has collaborated with esteemed colleagues, including Fred W Wagner and Sheldon M Schuster. These partnerships have further enriched his research and innovation efforts.
Conclusion
Dwane E Wylie stands out as a significant figure in the realm of scientific innovation, particularly in protein purification and metal binding technologies. His contributions continue to influence the scientific community and pave the way for future advancements.