The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 10, 1993
Filed:
Jun. 28, 1990
Fred W Wagner, Walton, NE (US);
Thomas R Coolidge, Falls Village, CT (US);
Sheldon M Schuster, Gainesville, FL (US);
Jay Stout, Lincoln, NE (US);
Dwane E Wylie, Lincoln, NE (US);
Klaus Breddam, Glostrup, DK;
William Lewis, Lincoln, NE (US);
Board of Regents of the University of Nebraska, Lincoln, NE (US);
BioNebraska, Inc., Lincoln, NE (US);
Abstract
An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein.