This inventor holds 6 USPTO granted patents. Top assignees: Children's Medical Center Corporation, Stratagene California, Stratagene. Active years: 1998-2007.
Location History:
- Dunwoody, GA (US) (1999 - 2006)
- Cambridge, MA (US) (1998 - 2007)
Company Filing History:
3 out of 1,071 
2 out of 44 
1 out of 84 
Years Active: 1998-2007
Title: Dowain A Wright: Innovator in Site-Directed Mutagenesis
Introduction
Dowain A Wright is a notable inventor based in Cambridge, MA (US). He has made significant contributions to the field of molecular biology, particularly in the area of site-directed mutagenesis. With a total of 6 patents to his name, Wright's work has advanced the methods used to manipulate DNA for various applications.
Latest Patents
One of Wright's latest patents is focused on circular site-directed mutagenesis. This invention provides improved methods for introducing site-directed mutations into circular DNA molecules using mutagenic primer pairs. The mutagenic primer pairs are selected to be either completely or partially complementary to each other, with the mutation site located within the region of complementarity. The process involves annealing a mutagenic primer pair to opposite strands of a circular DNA molecule, followed by synthesizing mutagenized DNA strands through a linear cyclic amplification reaction. After this synthesis step, a selection enzyme digests the parental template strands, resulting in the formation of a double-stranded circular DNA intermediate. This intermediate can then be transformed into suitable competent host cells, allowing for the recovery of closed circular double-stranded DNA that contains the desired mutations. Additionally, the invention includes kits for site-directed mutagenesis in accordance with these methods.
Career Highlights
Throughout his career, Dowain A Wright has worked with various organizations, including the Children's Medical Center Corporation. His innovative approaches have contributed to advancements in genetic engineering and molecular biology.
Collaborations
Wright has collaborated with notable individuals in his field, including John C Bauer and Jeffrey Carl Braman. These collaborations have likely enriched his research and development efforts.
Conclusion
Dowain A Wright's contributions to the field of site-directed mutagenesis exemplify his innovative spirit and dedication to advancing molecular biology. His patents and collaborations reflect a commitment to improving genetic manipulation techniques, which have far-reaching implications in research and medicine.