Dougalas C Youvan

Title: Dougalas C Youvan: Innovator in Fluorescence Microscopy

Introduction

Dougalas C Youvan is a prominent inventor based in San Diego, CA. He has made significant contributions to the field of microscopy, particularly in the area of fluorescence resonance energy transfer (FRET). His innovative work has led to advancements in imaging techniques that enhance the visualization and quantification of molecular interactions.

Latest Patents

Dougalas C Youvan holds 1 patent for his invention titled "Calibration of fluorescence resonance energy transfer in microscopy." This patent describes a system that provides imaging hardware, software, calibrants, and methods to visualize and quantify the amount of FRET occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for spectral overlap among donor, acceptor, and FRET spectra using well-characterized fluorescent beads as standards, along with radiometrically calibrated image processing techniques. Additionally, the system includes precisely machined epifluorescence cubes to ensure proper image registration during illumination at the donor and acceptor excitation wavelengths. Algorithms are also described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green), and FRET (red). The method has been demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

Career Highlights

Dougalas C Youvan is associated with Kairos Scientific Inc., where he continues to develop and refine his innovative technologies. His work has been instrumental in advancing the capabilities of fluorescence microscopy, making it a valuable tool for researchers in various scientific fields.

Collaborations

He has collaborated with notable colleagues, including Christopher M Silva and Edward J Bylina, contributing to the advancement of microscopy techniques and applications.

Conclusion

Dougalas C Youvan's contributions to fluorescence microscopy through his innovative patent have significantly impacted the field. His work continues to inspire advancements in imaging technologies that enhance our understanding of molecular interactions.