Diane E Wiffen

Title: Diane E Wiffen: Innovator in Hemoglobin Purification

Introduction

Diane E Wiffen is a notable inventor based in Georgetown, CA. She has made significant contributions to the field of hemoglobin purification, holding 2 patents that showcase her innovative approaches to this complex process.

Latest Patents

Her latest patents include methods for the purification of hemoglobin by displacement chromatography. In one patent, a preselected hemoglobin species is separated from contaminants with different acidity levels through an overload displacement chromatography process. This method utilizes anion exchange conditions to remove more acidic contaminants and cation exchange conditions for more basic contaminants. The exchange column is overloaded to displace the hemoglobin species with contaminants that have a greater affinity for the column, using the impure hemoglobin solution as the displacer. Another patent describes a two-stage displacement chromatography process that purifies hemoglobin from a crude solution, achieving an aqueous solution containing at least 99% of a preselected hemoglobin species. The first stage is conducted under anionic exchange conditions, followed by a cationic exchange process, ensuring a substantially pure hemoglobin solution.

Career Highlights

Diane E Wiffen is associated with Hemosol Inc., where she applies her expertise in hemoglobin purification. Her work has been instrumental in advancing the methods used in this field, contributing to better outcomes in medical applications.

Collaborations

Some of her notable coworkers include Diana H Pliura and Salman Ashraf, who collaborate with her on various projects within the company.

Conclusion

Diane E Wiffen's innovative work in hemoglobin purification has led to significant advancements in the field, demonstrating her commitment to improving medical technologies. Her contributions continue to impact the industry positively.