David Löper

Title: David Löper and a Phenol-Free Method for Isolating RNA

Introduction

David Löper is an inventor associated with Hilden, Germany, and Qiagen GmbH. His patent work includes a method designed to isolate RNA with high yield without using phenol. The available information lists 1 patent for him.

Latest Patents

Löper’s listed patent, “Method for Isolating RNA with High Yield,” describes a phenol-free process for isolating nucleic acids from a sample. It begins by adding a metal cation precipitant and an organic solvent to create an acidic, buffered precipitation mixture. The solvent concentration is 15% or less, and the mixture is used to precipitate proteins.

After the precipitate is separated, the supernatant contains small RNA shorter than 200 nucleotides and large RNA of at least 1,000 nucleotides. The nucleic acid is then isolated from the supernatant. According to the patent description, this approach can retain both small and large RNA, giving users flexibility to isolate and analyze different RNA targets.

Career Highlights

The supplied information associates Löper with Qiagen GmbH and identifies his work on RNA isolation as a patent contribution. The method focuses on protein precipitation conditions intended to support the recovery of RNA across different size ranges.

Collaborations

Kerstin Steinert is identified as Löper’s coworker in connection with the supplied information. No further details about their specific roles or collaboration are provided.

Conclusion

David Löper’s listed patent presents a phenol-free RNA isolation method that aims to recover both small and large RNA, offering flexibility for downstream analysis.