Company Filing History:
Years Active: 1998
Title: The Innovations of David James Wright
Introduction
David James Wright is a notable inventor based in Carrboro, NC (US). He has made significant contributions to the field of biotechnology, particularly in the development of innovative amplification methods. His work has implications for various applications in molecular biology and diagnostics.
Latest Patents
David James Wright holds a patent for "Strand displacement amplification using thermophilic enzymes." This patent describes a method known as Strand Displacement Amplification (SDA) that can be performed over a broad temperature range from 37°C to 70°C. The preferred temperature range for thermophilic SDA is between 50°C and 70°C. His research has shown that certain thermophilic restriction endonucleases can effectively nick the hemimodified restriction endonuclease recognition/cleavage site, which is essential for SDA. Additionally, specific thermophilic polymerases can extend from the nick while displacing the downstream strand. This innovative approach improves specificity and efficiency, reduces nonspecific background amplification, and potentially enhances the yields of amplification products. Notably, the need to add enzymes in a separate step after the initial heat denaturation of double-stranded targets is eliminated when using enzymes that can tolerate the denaturation temperature.
Career Highlights
David James Wright is currently employed at Becton Dickinson and Company Limited, where he continues to advance his research in biotechnology. His work has been instrumental in developing methods that enhance the efficiency of molecular amplification techniques.
Collaborations
Throughout his career, David has collaborated with several talented individuals, including Melinda S. Fraiser and Catherine A. Spargo. These collaborations have contributed to the success of his innovative projects and have fostered a productive research environment.
Conclusion
David James Wright's contributions to the field of biotechnology, particularly through his patent on thermophilic SDA, highlight his role as an influential inventor. His work continues to pave the way for advancements in molecular biology and diagnostics.