David Girata

Title: David Girata: Innovator in DNA Purification Methods

Introduction

David Girata is a notable inventor based in Redwood City, California. He has made significant contributions to the field of molecular biology, particularly in the area of DNA purification. With a total of 2 patents, his work focuses on innovative methods for isolating and analyzing circular DNA.

Latest Patents

David Girata's latest patents include methods for rapid separation and purification of DNA topological forms. These methods provide a novel approach for the isolation and analysis of circular DNA from complex samples, based on the topology of the DNA molecule. A sample comprising DNA species is combined with a chaotropic dense salt solution. A fraction containing the circular DNA of interest is isolated and dialyzed to remove excess salt. In some embodiments, salt gradients are generated by ultracentrifugation in the absence of intercalating dyes, such as ethidium bromide, and in the absence of protease digestion. The circular DNA thus isolated is substantially pure, with greater than about 75%, 80%, 90%, or even 95% of DNA in the isolated fraction comprised of circular DNA.

Career Highlights

Throughout his career, David Girata has worked with prestigious institutions, including Leland Stanford Junior University and the University of Texas System. His experience in these organizations has allowed him to develop and refine his innovative techniques in DNA purification.

Collaborations

David has collaborated with notable colleagues, including Massa Shoura and Stephen Levene. These partnerships have contributed to the advancement of his research and the successful development of his patented methods.

Conclusion

David Girata is a prominent figure in the field of DNA purification, with innovative methods that enhance the isolation and analysis of circular DNA. His contributions are significant in advancing molecular biology research and applications.