Company Filing History:
Years Active: 2019
Title: Chiara Stringari: Innovator in Fluorescence Lifetime Microscopy
Introduction
Chiara Stringari is a prominent inventor based in Irvine, CA (US). She has made significant contributions to the field of fluorescence lifetime microscopy, particularly in understanding the metabolic states of cells in living tissues. Her innovative work has the potential to advance research in stem cell metabolism and differentiation.
Latest Patents
Chiara Stringari holds a patent for a groundbreaking method titled "Phasor method to fluorescence lifetime microscopy to discriminate metabolic state of cells in living tissue." This label-free imaging technique allows for the monitoring of stem cell metabolism, enabling the discrimination of different states of stem cells as they differentiate within living tissues. The method utilizes intrinsic fluorescence biomarkers and the phasor approach to Fluorescence Lifetime Imaging Microscopy (FLIM). It identifies and maps intrinsic fluorophores such as collagen, retinol, retinoic acid, flavins, nicotinamide adenine dinucleotide (NADH), and porphyrin. The phasor values of germ cells in the germ line are measured, revealing their metabolic fingerprint, which clusters according to their differentiation state. This reflects changes in FAD concentration and NADH binding during the differentiation pathway. The phasor approach provides a label-free, fit-free, and sensitive method to identify different metabolic states of cells during differentiation, allowing for the detection of small changes in the redox state of cells and the prediction of cell fate.
Career Highlights
Chiara Stringari is affiliated with the University of California, where she continues her research and development in the field of microscopy and cell metabolism. Her work has garnered attention for its innovative approach and practical applications in biological research.
Collaborations
Chiara collaborates with esteemed colleagues, including Enrico Gratton and Michelle A. Digman, who contribute to her research endeavors and enhance the impact of her work in the scientific community.
Conclusion
Chiara Stringari's contributions to fluorescence lifetime microscopy represent a significant advancement in the understanding of cellular metabolism and differentiation. Her innovative patent and ongoing research continue to influence the field and pave the way for future discoveries.