Carl Preston Linn

Title: Innovations of Carl Preston Linn in Nucleic Acid Detection

Introduction

Carl Preston Linn is an accomplished inventor based in Durham, NC (US). He has made significant contributions to the field of nucleic acid detection, holding 2 patents that showcase his innovative approaches. His work has implications for various applications in biotechnology and molecular biology.

Latest Patents

Linn's latest patents include a method for the detection of nucleic acid targets by amplification. This method utilizes fluorescence polarization techniques for detecting nucleic acid amplification at thermophilic temperatures. The inventive approach employs a fluorescently labeled oligonucleotide signal primer, which transitions from a single- to double-stranded form in a target amplification-dependent manner. This conformational change results in an increase in fluorescence polarization values. Notably, the decrease in fluorescence polarization typically observed for duplexes at elevated temperatures is mitigated by double-stranded DNA binding proteins. These proteins stabilize the double-stranded structure by reducing the single-strandedness associated with higher temperatures. The methods developed by Linn provide a closed, homogeneous system for real-time amplification and detection of nucleic acids.

Another significant patent focuses on fluorescence polarization detection of nucleic acids. This invention offers methods for detecting both amplified and unamplified nucleic acid target sequences at increased temperatures through changes in fluorescence polarization. The decrease in fluorescence polarization linked to oligonucleotide hybridization at higher temperatures is addressed by incorporating a double-stranded DNA binding protein into the assay. At elevated temperatures, this protein not only restores but often enhances the magnitude of the fluorescence polarization change associated with the conversion of an oligonucleotide probe or primer from single- to double-stranded form.

Career Highlights

Carl Preston Linn is currently associated with Becton Dickinson and Company Limited, where he continues to advance his research in nucleic acid detection technologies. His work has been instrumental in developing methods that enhance the accuracy and efficiency of nucleic acid testing.

Collaborations

Linn has collaborated with notable colleagues, including G Terrance Walker and Patricia A Spears, contributing to the advancement of their shared research goals.

Conclusion

Carl Preston Linn's innovative contributions to nucleic acid detection highlight his expertise and commitment to advancing biotechnology. His patents reflect a deep understanding of molecular interactions and provide valuable tools for researchers in the field.