Can Shi

Title: Innovations in Genetic Research: The Contributions of Inventor Can Shi

Introduction

Can Shi is a prominent inventor based in Shanghai, China, recognized for his innovative contributions to the field of genetic research. With a focus on zebrafish mutants, Shi has advanced our understanding of biological functions through groundbreaking methodologies.

Latest Patents

Shi holds a patent titled "Method of preparing ddx27-deletion zebrafish mutants." This innovative method involves several steps: determining a target for ddx27 knockout on the sixth exon of the ddx27 gene in zebrafish, designing a gRNA sequence, and using specific primers for PCR amplification with a pUC19-gRNA scaffold plasmid as a template. The process also includes purifying and transcribing the PCR product in vitro to produce gRNA, introducing the gRNA and a Cas9 protein into the zebrafish, and culturing the zebrafish to obtain a mutant with stable inheritance. The application notably discloses the phenotype of the ddx27-deletion zebrafish mutant, which plays an essential role in investigating biological functions.

Career Highlights

Can Shi is affiliated with Shanghai Ocean University, where he contributes his expertise in genetics and innovation. His research at the university has positioned him as a leader in the study of genetic mutations, specifically through the application of cutting-edge techniques in molecular biology.

Collaborations

Shi collaborates closely with his coworker, Qinghua Zhang, further enriching the research impact within their field. Their joint efforts exemplify the importance of teamwork in advancing scientific understanding and innovation.

Conclusion

In summary, Can Shi's innovative work in developing methods for creating zebrafish mutants has significant implications for genetic research. His dedication and collaborations highlight the critical role of inventors in pushing the boundaries of science and enhancing our comprehension of biological functions.