Bruce E Lehnert

Title: Bruce E Lehnert: Innovator in Anthrax Research

Introduction

Bruce E Lehnert is a notable inventor based in Los Alamos, NM (US). He has made significant contributions to the field of anthrax research, holding 2 patents that focus on innovative methods for screening inhibitors of anthrax toxicity.

Latest Patents

One of his latest patents is titled "Method for screening inhibitors of the toxicity of Bacillus anthracis." This patent describes the protective antigen (PA) of Bacillus anthracis, which is integral to the mechanism of anthrax poisoning. The cloning, expression, and purification of a 32 kDa PA fragment (PA32) are detailed in this patent. This fragment has also been expressed as a fusion construct to stabilize green fluorescent protein (EGFP-PA32). Both proteins were capable of binding to specific cell surface receptors, as determined by fluorescent microscopy and a flow cytometric assay. To confirm binding specificity in the flow cytometric assay, non-fluorescent PA83 or PA32 was used to competitively inhibit fluorescent EGFP-PA32 binding to cell receptors. This assay can be employed as a rapid screen for compounds that disrupt the binding of PA to cells. Additionally, the high intracellular expression levels and ease of purification make this recombinant protein an attractive vaccine candidate or therapeutic treatment for anthrax poisoning.

Another significant patent is for a "Recombinant fluorescent protein microsphere calibration standard." This patent outlines a method for making recombinant fluorescent protein standard particles for the calibration of fluorescence instruments.

Career Highlights

Bruce E Lehnert is affiliated with the University of California, where he continues to contribute to research and innovation in his field. His work has implications for public health and safety, particularly in the context of bioterrorism and infectious diseases.

Collaborations

He has collaborated with notable colleagues, including Nick M Cirino and Paul J Jackson, who have also contributed to advancements in related research areas.

Conclusion

Bruce E Lehnert's innovative work in anthrax research and his patents reflect his commitment to advancing scientific knowledge and public health. His contributions are vital in the ongoing fight against infectious diseases.