Bo Cai

Title: Bo Cai and DNA-Linked Ligand Screening

Introduction

Bo Cai is an inventor associated with Lafayette, Indiana, and Purdue Research Foundation. His patent work addresses methods for screening small molecules and measuring their interactions with protein targets.

Latest Patents

Cai is associated with 1 patent: “Affinity Labeling of DNA-Linked Ligands for High Throughput Ligand Binding Assays and the Uses Thereof.” The disclosed method uses affinity crosslinking to connect DNA-linked ligands with proteins, supporting small-molecule screening and the measurement of apparent ligand affinity.

The patent describes applying the method to determine dissociation constants for 96 compounds against a protein target simultaneously and to measure a compound’s inhibitory activity against five protein targets in crude cell lysates. It also reports screening the Library of Pharmacologically Active Compounds against dihydrofolate reductase, leading to the identification of a novel inhibitor with an IC50 of 7.9 μM. An assay kit and its uses are also included in the disclosure.

Career Highlights

Cai’s patent reflects an approach designed to make protein–ligand testing more efficient by enabling parallel measurements and screening in complex biological samples. The disclosed applications include compound characterization and the identification of potential inhibitors.

Collaborations

Cai’s listed coworker is Casey J. Krusemark. The patent is associated with Purdue Research Foundation.

Conclusion

Bo Cai’s patent focuses on DNA-linked ligand assays that support high-throughput screening and protein-binding analysis, with applications in identifying and evaluating small-molecule inhibitors.