Company Filing History:
Years Active: 1999-2004
Title: Innovations in Cryopreservation by Bin Yu
Introduction
Bin Yu is an accomplished inventor based in Ithaca, NY (US). He has made significant contributions to the field of plant cell cryopreservation, holding 2 patents that focus on innovative methods for preserving plant cells.
Latest Patents
His latest patents detail methods for cryopreserving plant cells and recovering viable cells from both long and short-term cryopreservation. The process involves growing plant cells in culture and pretreating them with a solution containing a cryoprotective agent and a stabilizer. The pretreated cells are acclimated to reduced temperatures and loaded with agents such as DMSO, propylene glycol, or polyethylene glycol. These loaded cells are then incubated with a vitrification solution, which allows them to retain less than about 20% water content. This enables the cells to be frozen at cryopreservation temperatures for extended periods without significantly altering their genotypic or phenotypic characteristics. Additionally, the invention includes methods for lyophilizing cells to a preferable water content before exposure to a vitrification solution, effectively removing about 75% to 95% of the plant cell's water. The recovery process involves thawing the cells to room temperature and incubating them in a medium containing a cryoprotective agent and a stabilizer, allowing for successful recovery in liquid or semi-solid growth medium.
Career Highlights
Bin Yu is currently associated with Phyton Corporation, where he continues to advance research in plant cell preservation techniques. His work has the potential to significantly impact agricultural practices and biotechnology.
Collaborations
He has collaborated with notable colleagues such as Prakash G Kadkade and Christopher B Bare, contributing to the advancement of knowledge in his field.
Conclusion
Bin Yu's innovative approaches to cryopreservation are paving the way for new possibilities in plant biotechnology. His contributions are essential for the future of agricultural sustainability and research.