Company Filing History:
Years Active: 2006-2012
Title: Innovations in Oligonucleotide Synthesis by Andreas Wolter
Introduction
Andreas Wolter is a prominent inventor based in Hamburg, Germany. He has made significant contributions to the field of oligonucleotide synthesis, holding a total of six patents. His work focuses on improving the methods and compositions used in the synthesis of oligonucleotides, which are essential for various applications in biotechnology and medicine.
Latest Patents
One of his latest patents is titled "Process for the synthesis of oligonucleotides." This invention discloses novel methods for synthesizing oligonucleotides using nucleoside phosphoramidites on solid supports. The methods involve a stepwise chain assembly of oligonucleotides with 5'-acyl phosphoramidites, enhancing the quality of synthetic oligonucleotides. The synthesis cycles include a deprotection step, a phosphoramidite coupling step, a phosphite oxidation step, and an optional capping step. These innovations prevent the formation of deletion sequences and avoid acidic reagents that can lead to depurination side products.
Another significant patent is focused on "Activators for oligonucleotide and phosphoramidite synthesis." This invention introduces novel methods utilizing aryl-substituted 5-phenyl-1H-tetrazoles with perfluoroalkyl groups as activators. These activators are highly efficient, allowing for very short coupling times in the synthesis of oligonucleotides. They also provide pure phosphoramidites under mild conditions, characterized by their high solubility and non-hazardous nature.
Career Highlights
Andreas Wolter is currently associated with Sigma-Aldrich Co. LLC, where he continues to innovate in the field of oligonucleotide synthesis. His work has significantly advanced the methodologies used in this area, making him a key figure in the biotechnology sector.
Collaborations
He has collaborated with notable colleagues, including Michael Leuck and Wolfgang A Pieken, contributing to the advancement of research and development in oligonucleotide synthesis.
Conclusion
Andreas Wolter's contributions to the field of oligonucleotide synthesis through his innovative patents have made a lasting impact on biotechnology. His work continues to pave the way for advancements in the synthesis of oligonucleotides, enhancing their quality and efficiency.