Abhijit Guha

Title: Abhijit Guha: Innovator in G Protein Research

Introduction

Abhijit Guha is a notable inventor based in Etobicoke, Canada. He has made significant contributions to the field of biochemistry, particularly in the study of G proteins. His innovative methods have advanced the understanding of cellular processes and their implications in various diseases.

Latest Patents

Abhijit Guha holds a patent for a method of quantitating GTP and GDP bound to a G protein. This invention features methods for measuring the total amounts of G protein-bound GDP and GTP in mammalian cells and tissue. The method is advantageous over conventional techniques as it does not require cells to be exposed to phosphate-free growth conditions, which can negatively impact the accuracy of GTP determination. Additionally, it eliminates the need for radiolabelled phosphate in the growth medium. Standardized amounts of G protein-bound GDP and GTP are determined relative to non-nuclear cellular protein content or cellular DNA content, allowing for comparisons across different cell types. This method is particularly applicable to the detection of Ras protein bound GTP and GDP. The determination of Ras-bound GTP levels provides a sensitive and accurate means of evaluating the role of Ras activation in various cellular processes, including cell proliferative disease, hormonal secretion, cellular differentiation, programmed cell death, cell cycle regulation, and protein trafficking. This innovation allows for more precise targeting of treatments for abnormal processes related to G protein activation.

Career Highlights

Throughout his career, Abhijit Guha has worked with esteemed organizations such as Mount Sinai Hospital Corporation and the University of California. His work in these institutions has contributed to significant advancements in the understanding of G protein functions and their implications in health and disease.

Collaborations

Abhijit Guha has collaborated with notable colleagues, including Gerry R Boss and Jurgen S Scheele. These collaborations have further enriched his research and contributed to the development of innovative methodologies in the field.

Conclusion

Abhijit Guha's contributions to the field of biochemistry, particularly through his patented methods for quantitating GTP and GDP bound to G proteins, have had a profound impact on the understanding of cellular processes. His work continues to influence research and treatment strategies in various medical fields.