The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 05, 2018

Filed:

Oct. 10, 2013
Applicants:

Board of Regents of the University of Texas System, Austin, TX (US);

AM Biotechnologies, Llc, Houston, TX (US);

Inventors:

David G Gorenstein, Houston, TX (US);

Weiguo He, Pearland, TX (US);

David E Volk, Dickinson, TX (US);

Miguel-Angel Elizondo-Riojas, Houston, TX (US);

Ross Durland, The Woodlands, TX (US);

Johnnie Engelhardt, West Columbia, TX (US);

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/115 (2010.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1058 (2013.01); C12N 15/1048 (2013.01); C12N 15/115 (2013.01); C12N 2310/16 (2013.01); C12N 2310/315 (2013.01); C12N 2310/351 (2013.01); C12N 2320/11 (2013.01); C12N 2330/31 (2013.01);
Abstract

Provided herein are methods for a novel bead-based next-generation 'X-aptamer' selection scheme that extends aptamer technology to include X-modified bases, thus resulting in X-aptamers, at any position along the sequence because the aptamers are chemically synthesized via a split-pool scheme on individual beads. Also provides are application to a wide range of commonly used DNA modifications, including, but not limited to, monothioate and dithioate backbone substitutions. This new class of aptamer allows chemical modifications introduced to any of the bases in the aptamer sequence as well as the phosphate backbones and can be extended to other carbohydrate-based systems.


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