The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 20, 2018

Filed:

Jun. 12, 2014
Applicant:

Seikagaku Corporation, Tokyo, JP;

Inventors:

Hideo Mochizuki, Tokyo, JP;

Kiwamu Yamagishi, Tokyo, JP;

Kiyoshi Suzuki, Tokyo, JP;

Yeong Shik Kim, Seoul, KR;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/24 (2006.01); C12N 9/90 (2006.01); C08B 37/00 (2006.01); C12P 19/04 (2006.01);
U.S. Cl.
CPC ...
C12P 19/24 (2013.01); C08B 37/0075 (2013.01); C12N 9/90 (2013.01); C12P 19/04 (2013.01); C12Y 501/03 (2013.01);
Abstract

To provide a polypeptide having heparosan-glucuronate 5-epimerase activity, whereby means for producing a polysaccharide in which hexuronic acid residues has been epimerized is provided. Through screening ofcDNA library, a DNA encoding a polypeptide of heparosan-glucuronate 5-epimerase is obtained. The epimerase acts on glucuronic acid residues of N-acetyl heparosan and/or iduronic acid residues of completely desulfated N-acetylated heparin. The polypeptide encoded by the DNA is expressed by insect cells, to thereby yield the polypeptide having heparosan-glucuronate 5-epimerase activity. By bringing the polypeptide into contact with N-acetyl heparosan or completely desulfated N-acetylated heparin, a polysaccharide in which hexuronic acid residues has been epimerized is yielded.


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