The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 23, 2017
Filed:
Jun. 17, 2011
Stanley B. Prusiner, San Francisco, CA (US);
John C. Price, Hayward, CA (US);
Sina Ghaemmaghami, San Francisco, CA (US);
AL Burlingame, Sausalito, CA (US);
Shenheng Guan, Palo Alto, CA (US);
Stanley B. Prusiner, San Francisco, CA (US);
John C. Price, Hayward, CA (US);
Sina Ghaemmaghami, San Francisco, CA (US);
Al Burlingame, Sausalito, CA (US);
Shenheng Guan, Palo Alto, CA (US);
The Regents of the University of California, Oakland, CA (US);
Abstract
An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins. Tissue samples are removed from the subject at different points in time and proteins are extracted and separated so that different proteins of different tissues can be individually analyzed and their amount and pattern of isotopic labeling can be determined. In a preferred embodiment, the methodology can be combined with proteolytic digestion to peptides and analysis by mass spectrometry in order to measure rates of protein turnover in vivo relating to thousands of different proteins.