The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 04, 2016

Filed:

Feb. 06, 2014
Applicant:

The Trustees of Princeton University, Princeton, NJ (US);

Inventors:

Ron Weiss, Princeton, NJ (US);

Ihor Lemischka, Princeton, NJ (US);

Priscilla Purnick, West Windsor, NJ (US);

Christoph Schaniel, New York, NY (US);

Miles Miller, Indianapolis, IN (US);

Patrick Guye, Rupperswil, CH;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 15/11 (2006.01); C12N 15/09 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C12N 2800/40 (2013.01); C12N 2830/002 (2013.01); C12N 2830/003 (2013.01); C12N 2830/005 (2013.01); C12N 2830/006 (2013.01); C12N 2830/008 (2013.01); C12N 2830/15 (2013.01); C12N 2830/55 (2013.01); C12N 2830/85 (2013.01);
Abstract

The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.


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