The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 14, 2016

Filed:

Dec. 04, 2014
Applicant:

Danisco Us Inc., Palo Alto, CA (US);

Inventors:

Jing Ge, Shanghai, CN;

Ling Hua, Shanghai, CN;

Martijn Silvan Scheffers, Leiden, NL;

Zhongmei Tang, Shanghai, CN;

Marco Van Brussel-Zwijnen, Zoetermeer, NL;

Casper Vroemen, Oegstgeest, NL;

Bo Zhang, Shanghai, CN;

Kathleen A. Clarkson, San Francisco, CA (US);

Jacquelyn A. Huitink, Burlingame, CA (US);

Paula Johanna Maria Teunissen, Saratoga, CA (US);

Assignee:

Danisco US Inc., Palo Alto, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/14 (2006.01); C12C 7/04 (2006.01); C12P 7/14 (2006.01);
U.S. Cl.
CPC ...
C12P 7/14 (2013.01); C12C 7/04 (2013.01); C12P 19/14 (2013.01); C12P 2201/00 (2013.01);
Abstract

A fungal α-amylase is provided from(AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.


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