The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 02, 2016
Filed:
Feb. 16, 2010
Marcel P. Bruchez, Pittsburgh, PA (US);
Lauren A. Ernst, Pittsburgh, PA (US);
James Fitzpatrick, La Jolla, CA (US);
Chris Szent-gyorgyi, Pittsburgh, PA (US);
Brigitte F. Schmidt, Pittsburgh, PA (US);
Alan Waggoner, Pittsburgh, PA (US);
Marcel P. Bruchez, Pittsburgh, PA (US);
Lauren A. Ernst, Pittsburgh, PA (US);
James Fitzpatrick, La Jolla, CA (US);
Chris Szent-Gyorgyi, Pittsburgh, PA (US);
Brigitte F. Schmidt, Pittsburgh, PA (US);
Alan Waggoner, Pittsburgh, PA (US);
Carnegie Mellon University, Pittsburgh, PA (US);
Abstract
The present invention presents designs for high extinction quenched 'dyedrons' that can be activated by conversion of a single acceptor/quencher in the molecular assembly to a fluorescent state. The quencher is activated by noncovalent binding to a unique complementary expressible fluorogen activating peptide (FAP). In this way, the quencher serves as the homogeneous switch, receiving energy efficiently from each of the donor molecules of the dendronic antenna, and releasing it as fluorescence only when activated by binding. The sum of the extinction of the multiple dyes on the antenna will provide dramatic enhancements in the effective brightness of the probe in standard imaging systems. This approach provides a set of probes with exceptional brightness, specifically targeted to an expressed tag that activates the fluorescence of the dyedron.