The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 29, 2015
Filed:
Mar. 23, 2010
Peter Sebo, Prague, CZ;
Adriana Osickova, Prague, CZ;
Jiri Masin, Uvaly, CZ;
Catherine Fayolle, Epinay sur Orge, FR;
Jan Krusek, Prague, CZ;
Marek Basler, Prague, CZ;
Claude Leclerc, Paris, FR;
Radim Osicka, Prague, CZ;
Peter Sebo, Prague, CZ;
Adriana Osickova, Prague, CZ;
Jiri Masin, Uvaly, CZ;
Catherine Fayolle, Epinay sur Orge, FR;
Jan Krusek, Prague, CZ;
Marek Basler, Prague, CZ;
Claude Leclerc, Paris, FR;
Radim Osicka, Prague, CZ;
INSTITUT PASTEUR, Paris, FR;
INSTITUTE OF MICROBIOLOGY OF THE ASCR, V.V.I., Prague, CZ;
INSTITUTE OF PHYSIOLOGY OF THE ASCR. V.V.I., Prague, CZ;
Abstract
A polypeptide comprises a mutatedCyaA (SEQ ID NO:1),CyaA (SEQ ID NO:7),CyaA (SEQ ID NO:8), orCyaA (SEQ ID NO:9), or a fragment thereof lacking all or part of the N-terminal catalytic domain of the CyaA, or a polypeptide having at least 95% sequence identity with SEQ ID NOS: 1, 7, 8, or 9. The glutamic acid residue at position 570 of SEQ ID NO: 1, 7, 8 or at position 569 of SEQ ID NO:9 has been substituted by a conservative amino acid residue. The lysine residue at position 860 of SEQ ID NO: 1, 7, 8 or at position 859 of SEQ ID NO:9 has been substituted by a conservative amino acid residue. The polypeptide retains the binding activity of native CyaA to CD11b expressing cell, and has a pore-forming activity which is reduced or suppressed as compared to that of the native CyaA toxin.