The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 24, 2015

Filed:

Mar. 23, 2011
Applicants:

Harald Sobek, Penzberg, DE;

Johann-peter Thalhofer, Weilheim, DE;

Rainer Mueller, Penzberg, DE;

Manfred Schmidt, Penzberg, DE;

Michael Greif, Penzberg, DE;

Armin Ruf, Freiburg, DE;

Christian Rudolph, Penzberg, DE;

Inventors:

Harald Sobek, Penzberg, DE;

Johann-Peter Thalhofer, Weilheim, DE;

Rainer Mueller, Penzberg, DE;

Manfred Schmidt, Penzberg, DE;

Michael Greif, Penzberg, DE;

Armin Ruf, Freiburg, DE;

Christian Rudolph, Penzberg, DE;

Assignee:

Roche Diagnostics Operations, Inc., Indianapolis, IN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1247 (2013.01);
Abstract

The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.


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