The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 03, 2015
Filed:
Apr. 11, 2012
Janne Brunstedt, Roskilde, DK;
Jens Frisbæk Sorensen, Aarhus, DK;
Jørn Borch Søe, Tilst, DK;
Charlotte Johansen Vedel, Holte, DK;
Birgitte Ø. Wittschieben, Risskov, DK;
Jørn Dalgaard Mikkelsen, Hvidovre, DK;
Charlotte Horsmans Poulsen, Brabrand, DK;
Lene B. Jensen, Højbjerg, DK;
Lone B. Miller, Viby J, DK;
Morten K. Larsen, Sabro, DK;
Rikke H. Lorentzen, Randers, DK;
Charlotte R. Thoudahl, Greve, DK;
Marguerite A. Cervin, Redwood City, CA (US);
Richard R. Bott, Burlingame, CA (US);
Janne Brunstedt, Roskilde, DK;
Jens Frisbæk Sorensen, Aarhus, DK;
Jørn Borch Søe, Tilst, DK;
Charlotte Johansen Vedel, Holte, DK;
Birgitte Ø. Wittschieben, Risskov, DK;
Jørn Dalgaard Mikkelsen, Hvidovre, DK;
Charlotte Horsmans Poulsen, Brabrand, DK;
Lene B. Jensen, Højbjerg, DK;
Lone B. Miller, Viby J, DK;
Morten K. Larsen, Sabro, DK;
Rikke H. Lorentzen, Randers, DK;
Charlotte R. Thoudahl, Greve, DK;
Marguerite A. Cervin, Redwood City, CA (US);
Richard R. Bott, Burlingame, CA (US);
DUPONT NUTRITION BIOSCIENCES APS, Copenhagen, DK;
Abstract
The present invention provides a method for preparing a variant lipid acyltransferase enzyme by expressing a nucleotide sequence encoding a lipid acyltransferase which may comprise at least one modification at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6 in a host organism.