The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 28, 2015

Filed:

Nov. 14, 2012
Applicant:

Institut National DE LA Sante ET DE LA Recherche Medicale (Inserm), Paris, FR;

Inventors:

Didier Trono, Collonge, CH;

Francois-Loic Cosset, Lyons, FR;

Virginie Sandrin, Lyons, FR;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 7/00 (2006.01); C12N 15/86 (2006.01); C07K 14/005 (2006.01); C12N 7/04 (2006.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); C07K 14/005 (2013.01); A61K 48/00 (2013.01); C07K 2319/00 (2013.01); C07K 2319/03 (2013.01); C12N 7/045 (2013.01); C12N 2510/02 (2013.01); C12N 2740/13022 (2013.01); C12N 2740/13043 (2013.01); C12N 2740/15043 (2013.01); C12N 2740/15045 (2013.01); C12N 2810/60 (2013.01); C12N 2830/50 (2013.01);
Abstract

A method is provided for producing a recombinant pseudotyped viral vector particle wherein a cell is transfecting with (i) at least one vector construct; (ii) at least one packaging construct; and (iii) an expression construct encoding a chimeric glycoprotein encoded by a nucleic acid comprising the sequence of SEQ ID NO: 1 to yield a producer cell, followed by culturing the producer cell in a medium; and separating the producer cell from the medium to recover the recombinant viral vector particle from the medium. Vectors obtained in this manner have significantly higher titers than vectors coated with the parental non-chimeric glycoprotein.


Find Patent Forward Citations

Loading…