The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 31, 2015
Filed:
Aug. 28, 2007
Ted H. Hansen, North Potomac, MD (US);
Daved Fremont, Saint Louis, MO (US);
Janet Connolly, North Potomac, MD (US);
Lonnie Lybarger, Tucson, AZ (US);
Michael Miley, Cary, NC (US);
Vesselin Mitaksov, Ballwin, MO (US);
Steven Truscott, Royal Oak, MI (US);
Ted H. Hansen, North Potomac, MD (US);
Daved Fremont, Saint Louis, MO (US);
Janet Connolly, North Potomac, MD (US);
Lonnie Lybarger, Tucson, AZ (US);
Michael Miley, Cary, NC (US);
Vesselin Mitaksov, Ballwin, MO (US);
Steven Truscott, Royal Oak, MI (US);
Washington University, Saint Louis, MO (US);
Abstract
A disulfide trap, comprising an antigen peptide covalently attached to an MHC class I heavy chain molecule by a disulfide bond extending between two cysteines, is disclosed. In some configurations, a disulfide trap, such as a disulfide trap single chain trimer (dtSCT), can comprise a single contiguous polypeptide chain. Upon synthesis in a cell, a disulfide trap oxidizes properly in the ER, and can be recognized by T cells. In some configurations, a peptide moiety of a disulfide trap is not displaced by high-affinity competitor peptides, even if the peptide binds the heavy chain relatively weakly. In various configurations, a disulfide trap can be used for vaccination, to elicit CD8 T cells, and in multivalent MHC/peptide reagents for the enumeration and tracking of T cells. Also disclosed are nucleic acids comprising a sequence encoding a disulfide trap. Such nucleic acids, which can be DNA vectors, can be used as vaccines.